Comparison of Tissue Stages of Hepatozoon americanum in the Dog Using Immunohistochemical and Routine Histologic Methods
- Department of Pathobiology, College of Veterinary Medicine, Oklahoma State University, Stillwater, OK
- Dr. R. J. Panciera, College of Veterinary Medicine, Oklahoma State University, Stillwater, OK 74078 (USA)
Abstract
American canine hepatozoonosis is caused by Hepatozoon americanum, a recently described species of apicomplexan protozoan parasite. An immunohistochemical procedure using a polyclonal antibody to sporozoites of H. americanum clearly identified asexual stages of H. americanum in canine striated muscle. The method also detects hepatozoa present in naturally infected coyotes and raccoons and reacts with certain other apicomplexans. Use of this immunohistochemical procedure confirms the canine intermediate host-parasite relationships that were presumptively established using conventional histopathologic methods.
- © 2001 American College of Veterinary Pathologists
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